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Promix is a new type of chromatography stationary phase designed for separation of proteins and peptides. There are four types of phases that SIELC specialists developed for separation of this type of bio-molecule.

Promix SP is a column for very short and/or very hydrophilic peptides. Typically it is difficult to retain such peptides on RP column. The Promix SP column offers an improved retention profile and alternative selectivity if compared with RP separation. Promix AP is a column designed for short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. A pH gradient is the most convenient way to control retention of basic peptides on this column. Use a buffer with a neutral or slightly acidic pH (4.5 – 7) in the beginning of the gradient, and use a more acidic buffer (pH 2 – 3.5) in the end of the gradient elution. Promix MP is a column designed for medium size hydrophobic peptides. Typical applications include protein digest which increases peak capacity of the digest by 30-50%. This column offers alternative selectivity if compared with simple RP columns. Pairs of peptides that have been typically difficult to separate, can be resolved on this stationary phase.

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Protein/peptid selection

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