• Avantor ACE Oligo Columns Avantor® ACE® Oligo is a unique encapsulated stationary phase for high resolution ion-pair reversed-phase analysis of oligonucleotides.
Avantor® ACE® Oligo is a unique encapsulated stationary phase for high resolution ion-pair reversed-phase analysis of oligonucleotides. These high pH stable stainless steel columns are compatible with all buffers and mobile phases typically used for oligonucletide analysis by LC-UV and LC-MS, including TEAA and HFIP.

  • High efficiency 1,7 and 3,0 µm particle sizes for high resolution oligonucleotide separations
  • Ultra-inert, base deactivated silica for exceptional reproducibility and peak shape
  • Excellent column lifetime under oligonucleotide testing conditions
  • Ultra-low bleed for excellent LC/MS compatibility

The oligonucleotide UHPLC and HPLC bioanalytical columns from Avantor® ACE® offer excellent peak shape and have improved retention, which allow you to pull the first peak and any early eluting impurities away from your unretained analytes at t0. The Oligo columns are available in analytical, semipreparative, and preparative sizes (2.1, 3.0, 4.6, 10, and 21.2 mm IDs).

  • Proprietary encapsulated stationary phase for oligonucleotide analysis
  • Ultra-inert silica and high-efficiency 1.7- and 3-μm particle sizes for optimum peak shape and efficiency
  • Extended pH stability up to 11.5 with LC-MS compatible buffers
  • Highly reproducible stationary phase: batch-to-batch and column-to-column
  • Excellent column lifetime under oligonucleotide testing conditions

To extend column lifetime, ACE UHPLC guard cartridges (EXL-1P15-GD2U / EXL-1P15-GD4U) are recommended for 2.1- and 4.6-mm ID columns, respectively. The UHPLC guard cartridge holder (H0011) is required for both guard cartridge IDs.



Column: ACE Excel 3 Oligo
Dimensions: 150 x 4.6 mm
Mobile Phases: A: 80 mM triethylammonium acetate in H2O (pH 7.0)
B: 80 mM triethylammonium acetate in MeCN
Gradient: Time (mins) %B
0 12
30 15
36 15
36.5 12
66.5 12
System Dwell Volume: 550 μL
Flow Rate: 1.0 mL/min
Injection: 20 μL
Temperature: 60 °C
Detection: UV, 260 nm


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